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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which ends up in chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was identified to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important job in advertising cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be described. Two added splice variants had been claimed but thorough nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind specially to the focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.
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This manual outlines more achievable optimization of mobile and immunoassay parameters to read more guarantee the very best final results are...
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This guideline outlines additional achievable optimization of cellular and immunoassay parameters to be sure the absolute best success are...
Antibodies bound to the blocking peptide no more bind into the epitope on the goal protein. By comparing the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is certain.
Target Details Cyclin E belongs to your very conserved cyclin family members, whose associates are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which add on the temporal coordination of each mitotic celebration. Cyclin E types a posh with and functions being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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